Dr. Ramesh Subramanian
Over the years I have had opportunity to work in various laboratories where in I have gained expertise in range of molecular biological techniques. I have extensive experience in molecular biological techniques ranging from PCR, cloning, southern, northern and western blots. I also have expertise in primer design for Q-PCR and Q-PCR analysis, Codon optimization of coding sequences for enhanced expression, site-directed mutagenesis using PCR and genetic recombination techniques to generate mutant viruses. I have extensive experience in the design of sh RNAs for the specific targeting of mammalian genes using expression vectors. Furthermore I am experienced in DNA sequencing and analyzing sequences for mutations and codon optimizations. I am well equipped in the handling mammalian cell cultures suspension and adherent, virus infections, purification and concentration/purification of virus by step gradients. I have used purified virions for Immuno gold labeling experiments to demonstrate presence of envelope glycoproteins using Transmission Electron Microscopy. I am well versed with in vitro angiogenesis assays and generation of spheroids in vitro to carryout tumor migration studies as well as tumor studies on animal models.