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1. Biology of RNA and Next Generation Sequencing 4
Learn about the process of gene expression, alternative splicing and assays for mRNA detection and quantification
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Biology of Transcription 30 minLecture1.1
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Detection and Quantification of RNA 34 minLecture1.2
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NGS: Gene Expression Data 38 minLecture1.3
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Transcriptomics 1: Quiz 1 10 questionsQuiz1.1
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2. Processing RNA-seq data and generating a table of expression 3
Learn about Next Generation Sequencing and the steps to analyze and interpret RNA-seq data
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Introduction to an RNA-seq study 25 minLecture2.1
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The Logic of RNA-seq Analysis 01 hourLecture2.2
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Transcriptomics 1: Quiz 2 10 questionsQuiz2.1
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3. Understanding The Table of Expression 3
Learn about working with the table of expression to get a high-level view of RNA-seq data
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Working with your Gene Expression Table 35 minLecture3.1
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Biological Interpretation and Annotation 10 minLecture3.2
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Hands-on Assignment: Transcriptomics 1 10 daysAssignment3.1
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4. Conclusion and Additional Resources 3
Additional resources for reading to review what we learned in Transcriptomics 1
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Technical Details on Methods We Used 02 hour 30 minLecture4.1
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Conclusion and Review 40 minLecture4.2
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Transcriptomics 1 Course Feedback 10 daysAssignment4.1
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30 Comments
where are all the lessons?
You can check your top of the website page for the courses menu
How do I enroll?
There should be an enrollment button, but it appears to be missing for some users and certain courses. We are working on getting to the bottom of this issue..
issue fixed, you should be able to enroll now
This is simply awesome course. I loved this course. It made many of my concepts clear and the quiz was pretty exciting and challenging… looking forward to get enrolled in other courses too.
I recently took Transcriptomics 1 which helped me to clear my basics and gain an in-depth insight to what it is like to successfully handle data of any omics especially bioinformatics through excellent videos and quizzes. I look forward to utilizing the platform in research and gaining better knowledge of my core subjects.
This is really helpful data … I am very thankful for this
It’s very useful and help me to figure out my research project.
Thanks,
Glad to know that – hoping that your research project yields interesting results!
Transcriptomics 1 have helped me to get a clear knowledge about this topic and quiz is so interesting..
Interesting one.
I would suggest doing the (Principal Component Analysis) PCA course for the Interpretation of Output Data of Hands-on Project. and understand Transcriptomics 2.
Thank you!
I have started the assignment in transcriptomic 1 section 3.1 three days ago. My created pipeline (Test Run 1) has not yet finished and it is in its 50% processing step. Is there anything wrong?
There is nothing wrong, the pipeline run time is determined by data size, pipeline complexity and number of users simultaneously running analysis at the same time.
Extremely helpful course designed well to cover the basics on Transcriptomics which is a widely used technique that obtains information on the abundance of multiple mRNA transcripts within a biological sample simultaneously.There are various examples, pictorial representation to help us better comprehend the content. There are quizzes which are conceptual that will help you self access and get clarity over the subject. This course also allows you to work with hands on assignment where you can practically perform the analysis which helps in even better understanding of the subject on a whole. I would personally recommend it.
Is there a trouble-shooting page? I’ve been trying to run the RNA-seq pipeline, and it keeps getting hung up at 50% processing. I cannot get my results.
Each course has a forum for technical issues.
I was told that once we paid for a course that we would maintain access. Has this changed and we have to pay for a course a second time if we don’t complete it after a certain period of time?
We checked and your purchase was done via a subscription, which is a monthly fee. You can also purchase individual courses, those will not require to be purchased again until they are updated with new material (which happens, but rarely)
Hi can you please send me the certificate for this course as I can not access it?
you should be able to see your certificates under your profile: https://edu.t-bio.info/members/yana-gupta/courses/certificates/
Good day Sir, please why I grapple with the hands on assignment on transcriptomics 1, I started studying transcriptomics 2 contents,only to notice that my course progress didn’t increase at all,still o%, would that change to indicate all the progress I have made so far after in transcriptomics 2,after I get the correct answer? That was why I submitted earlier, to know if that will change, please how do I rectify this??
Dear Anthony, the course progress is only measured when you press “FINISH” (a button at the bottom of each lesson, or if you press “COMPLETE COURSE”
Good morning sir,
Please the section 4.1 on transcriptomics 1, keep logging me out automatically, I have not been able to assess its contents, or complete it, how can I correct this
Its “technical details on the method we used”
Sorry to hear that, please try again and let us know: support@pine.bio
This is simply amazing course!!! It provides a holistic approach to analyze RNA-seq raw data in the simplest manner. If someone is interested in learning the RNA-seq analysis in detail, one must take this course!!
I’m unable to upload the Breast_Cancer_Education1.svl file for transcriptomics 1 assignment. I keep getting server message: ”Please wait until the file is processed for use”.
Please refresh the server page and clear cache – I think everything is working, but if you are facing issues, you can email: support [at] pine.bio and post on the organization forum.
Hello Hyacinth, you have to wait until the file is uploaded and ready. For me, it takes a few seconds if I get that message after uploading the file.
This course was extremely helpful! It definitely gave me an insight on current bioinformatics technologies in the pipeline for RNA-seq analysis.