Day 1
High Throughput Data in Life Sciences: Pratim Chakraborty, July 26th, 2019
- The Central dogma of molecular biology: DNA code, Transcription, and Translation
- Measurement of molecular activity at the subcellular level: Quantification Techniques
- Next Generation Sequencing: DNA and RNA Analysis
Day 2
Next Generation Sequencing: Transcriptomics 1, Mohit Mazumder, July 29th, 2019
- Generating Transcriptome Expression Data
- Role of pre-processing in standard RNA-seq pipelines (Trimmomatic and PCR-clean)
- Mapping techniques: mapping on the transcriptome, mapping on genome and combined strategies (Bowtie, BWA and TopHat/HiSat)
- Quantification and Generating a table of expression: RSEM