Day 1

High Throughput Data in Life Sciences: Pratim Chakraborty, July 26th, 2019

• The Central dogma of molecular biology: DNA code, Transcription, and Translation
• Measurement of molecular activity at the subcellular level: Quantification Techniques
• Next Generation Sequencing: DNA and RNA Analysis

Day 2

Next Generation Sequencing: Transcriptomics 1, Mohit Mazumder, July 29th, 2019

• Generating Transcriptome Expression Data
• Role of pre-processing in standard RNA-seq pipelines (Trimmomatic and PCR-clean)
• Mapping techniques: mapping on the transcriptome, mapping on genome and combined strategies (Bowtie, BWA and TopHat/HiSat)
• Quantification and Generating a table of expression: RSEM