OmicsLogic Transcriptomics 2020

The OmicsLogic Transcriptomics program will introduce real-world applications of RNA-seq and provide participants with hands-on skills and logical background to the full RNA-seq analysis approach. We will review methods of quantitative and qualitative analysis of mRNA expression in a sample. Other sessions will focus on how data is generated using Next-Generation Sequencing. Practical sessions will guide participants to use the methods we review on several project datasets to practice generating a table of expression from raw FASTq files and perform subsequent analysis of this table of gene and isoform expression.

Upcoming Events

  1. 09
    December
    OmicsLogic Transcriptomics Program overview
    09:00 - 10:00
    Online

    Overview of the entire course, Overview of the tools and resources for this program (edu.t-bio.info courses, projects and datasets, and the T-BioInfo Analytics Platform),...

  2. 11
    December
    Processing of Gene expression NGS data
    09:00 - 10:00
    Online

    Role of pre-processing in standard RNA-seq pipelines (Trimmomatic and PCR-clean) Mapping techniques: mapping on the transcriptome, mapping on the genome and combined strategies (Bowtie,...

  3. 14
    December
    Exploring Gene Expression Tables and Statistical Analysis
    09:00 - 10:00
    Online

    Review Quantification and Generating a table of expression: RSEM, HTSeq, and Sailfish Statistical Analysis: Filtering, removing noise and Normalization Techniques Correlation – detecting correlation...

  4. 16
    December
    Introduction to Data Mining and Machine learning for RNA-seq data
    09:00 - 10:00
    Online

    Introduction to unsupervised machine learning  Dimensionality Reduction: PCA, H-Clust, K-means Introduction to Supervised Machine Learning  Predictive Analysis: Random Forest, SVM, LDA.  Feature Selection and...

  5. 18
    December
    Single-cell Transcriptomics
    09:00 - 10:00
    Online

    Single-cell transcriptomics (SCT) Introduction to SCT, History of SCT, NGS Techniques, Capture techniques, Quantification, scRNA-seq data preparation & Counts, ScRNA data analysis, Publication & projects:...

  6. 21
    December
    Project examples and publicly available RNA-Seq datasets
    09:00 - 10:00
    Online

    Planning your project,  Q&A sessions, Audio & Video Presentation Case Studies & Publications, Datasets

  7. 23
    December
    Transcriptomics Project feedback & Review
    09:00 - 10:00
    Online

    Review of Project Proposal, Expert feedback for peer review poster presentation

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Introduction to Bioinformatics

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