OmicsLogic Transcriptomics 2020
The OmicsLogic Transcriptomics program will introduce real-world applications of RNA-seq and provide participants with hands-on skills and logical background to the full RNA-seq analysis approach. We will review methods of quantitative and qualitative analysis of mRNA expression in a sample. Other sessions will focus on how data is generated using Next-Generation Sequencing. Practical sessions will guide participants to use the methods we review on several project datasets to practice generating a table of expression from raw FASTq files and perform subsequent analysis of this table of gene and isoform expression.
Upcoming Events
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15September
OmicsLogic Transcriptomics Program overview
Overview of the entire course, Overview of the tools and resources for this program (edu.t-bio.info courses, projects and datasets, and the T-BioInfo Analytics Platform),...
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16September
Processing of Gene expression NGS data
Role of pre-processing in standard RNA-seq pipelines (Trimmomatic and PCR-clean) Mapping techniques: mapping on the transcriptome, mapping on the genome and combined strategies (Bowtie,...
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18September
Exploring Gene Expression Tables and Statistical Analysis
Review Quantification and Generating a table of expression: RSEM, HTSeq, and Sailfish Statistical Analysis: Filtering, removing noise and Normalization Techniques Correlation – detecting correlation...
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22September
Introduction to Data Mining and Machine learning for RNA-seq data
Introduction to unsupervised machine learning Dimensionality Reduction: PCA, H-Clust, K-means Introduction to Supervised Machine Learning Predictive Analysis: Random Forest, SVM, LDA. Feature Selection and...
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23September
Single-cell Transcriptomics
Single-cell transcriptomics (SCT) Introduction to SCT, History of SCT, NGS Techniques, Capture techniques, Quantification, scRNA-seq data preparation & Counts, ScRNA data analysis, Publication & projects:...
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25September
Project examples and publicly available RNA-Seq datasets
Planning your project, Q&A sessions, Audio & Video Presentation Case Studies & Publications, Datasets
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06October
Transcriptomics Project feedback & Review
Review of Project Proposal, Expert feedback for peer review poster presentation
This is a private organization. To join you must be a registered site member and request organization membership.